QIAGEN

Ni-NTA Fast Start Kit

For purification and detection of recombinant His-tagged proteins from E. coli lysates

·         Ideal for researchers new to protein science

·         Everything required for efficient purification in one kit

·         Easy-to-follow protocols and straightforward processing

·         Up to 25 mg protein per column in as little as 90 minutes

The Ni-NTA Fast Start Kit provides everything needed for fast, efficient purification of His-tagged proteins from cleared E.coli lysates, including prefilled Ni-NTA columns.

Buffers supplied in the kit enable proteins to be purified either under native or denaturing conditions.

The kit also contains an Anti-His antibody for detection of expressed His-tagged proteins.

Feature	Specifications
Applications	Proteomics
Binding capacity	5-20 mg/ml
Gravity flow or spin column	Gravity flow
Processing	Manual
Special feature	Ni-NTA technology
Start material	Cell lysate
Support/matrix	Ni-NTA matrix
Tag	6xHis tag
Yield	<10 mg protein per column

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